Home built PickIt2 not behaving normally.

Hi All,
I am having a strange problem with a homebuilt PickIt2. It is wired just as MicroChip designed it, but I substituted a few parts to facilitate thru hole construction. I subbed a BS250P P channel MOSFET for the IRLML6402 and the Q2 part of U5, and a TN0702N3 for the Q1 part of U5. I also subbed a MCP6041 op amp for the MCP6001 op amp. The board is recognized by the PickIt2 v2.61 software. The problems are as follows:

1) When first plugged I get the error message bo that says "Pickit...

Home built PickIt2 not behaving normally.